Gene expression under laser and light-emitting diodes radiation for modulation of cell adhesion: Possible applications for biotechnology
✍ Scribed by Tiina Karu; Ludmila Pyatibrat
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 130 KB
- Volume
- 63
- Category
- Article
- ISSN
- 1521-6543
- DOI
- 10.1002/iub.514
No coin nor oath required. For personal study only.
✦ Synopsis
Experimental data about the modulation of adhesion and proliferation of anchorage-dependent HeLa cells with monochromatic or quasimonochromatic radiation in red to nearinfrared region are presented. Cell adhesion and proliferation can be increased by irradiation with light of certain wavelengths (maxima in action spectrum are 619, 675, 740, 760, and 820 nm) or decreased when the activity of photoacceptor (cytochrome c oxidase in mitochondrial respiratory chain) is inhibited by chemicals before the irradiation. This modality allows controlling the number of attached and/or proliferating cells. Possible biotechnology applications of this method are outlined. 2011 IUBMB IUBMB Life, 63(9): 747-753, 2011 Keywords modulation of anchorage-dependent cell adhesion and proliferation; monochromatic and quasimonochromatic red to near-IR radiation; retrograde mitochondrial signaling; charge-transfer and d-d transitions in Cu chromophores of cytochrome c oxidase molecule.