𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gene expression profiles in breast tumors regarding the presence or absence of estrogen and progesterone receptors

✍ Scribed by Maria Aparecida Nagai; Nancy da Rós; Mário Mourão Neto; Silvio Rodrigues de Faria Junior; Maria Mitzi Brentani; Roberto Hirata Jr.; Eduardo Jordão Neves

Publisher
John Wiley and Sons
Year
2004
Tongue
French
Weight
152 KB
Volume
111
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Estrogen acts via its receptor (ER) to stimulate cell growth and differentiation in the mammary gland. ER and progesterone receptor (PR), which is regulated by estrogen via ER, have been used as prognostic markers in clinical management of breast cancer patients. Patients with ER^−^ breast tumors have a poorer prognosis than patients with ER^+^ tumors. The aim of the present study was the identification of tumor‐associated genes differentially expressed in breast tumors regarding the presence or absence of ER and PR hybridized with cDNA microarrays containing 4,500 tumor‐derived expressed sequence tags generated using the ORESTES technique. Samples of human primary breast carcinomas from 38 patients were analyzed. The experiments were performed in triplicates and data from each element were acquired by phosphoimage scanning. Data acquisition was performed using the ArrayVision software. After normalization statistical analysis was applied. In a preliminary analysis, 98 differentially expressed transcripts were identified, 46 were found to be more expressed in ER^+^/PR^+^ and 52 were found to be more expressed in ER^−^/PR^−^ breast tumors. The biochemical functions of the genes in the reported expression profile are diverse and include metabolic enzymes, protein kinases, helicases, transcription factors, cell cycle regulators and apoptotic factors. ER^−^/PR^−^ breast tumors displayed increased levels of transcripts of genes associated with neurodegeneration and genes associated with proliferation were found in ER^+^/PR^+^ tumors. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Flow cytometric analysis of estrogen, pr
Flow cytometric analysis of estrogen, progesterone receptor expression and DNA content in formalin-fixed, paraffin-embedded human breast tumors
✍ Alka A. Redkar; Awtar Krishan 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 176 KB 👁 2 views

Flow cytometric analysis of estrogen (ER) and progesterone (PgR) receptor expression in archival human breast tumors is relatively difficult. We have used enzyme digestion and microwave antigen retrieval procedures for multiparametric flow cytometric analysis of ER and PgR expression and DNA content

Survival of premenopausal breast carcino
Survival of premenopausal breast carcinoma patients in relation to menstrual cycle timing of surgery and estrogen receptor/progesterone receptor status of the primary tumor
✍ Lucienne S. Cooper; Cheryl E. Gillett; Neera K. Patel; Diana M. Barnes; Ian S. F 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 98 KB 👁 3 views

## BACKGROUND. Premenopausal breast carcinoma patients who undergo tumor excision during the follicular phase of their menstrual cycle may have a significantly worse prognosis than those whose tumors are excised in other phases of the menstrual cycle. ## METHODS. Outcome was determined in a ser

Germ-line mutations in BRCA1 or BRCA2 in
Germ-line mutations in BRCA1 or BRCA2 in the normal breast are associated with altered expression of estrogen-responsive proteins and the predominance of progesterone receptor A
✍ Patricia A. Mote; Jennifer A. Leary; Kelly A. Avery; Kerstin Sandelin; Georgia C 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 177 KB 👁 1 views

## Abstract The breast cancer susceptibility genes __BRCA1__ and __BRCA2__ are responsible for a large proportion of familial breast and ovarian cancer, yet little is known of how disruptions in the functions of the proteins these genes encode increased cancer risk preferentially in hormone‐depende