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Gene expression and protein distribution of inter-α-trypsin inhibitor in three human hepatoma cell lines.

✍ Scribed by Antoine Héron; Hélène Borghi; Aleth Callé; Jeannette Bourguignon; Maryam Diarra-Mehrpour; Jean-Pierre Martin; Richard Sesboüé

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
740 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis

Abstract

In standard culture conditions, three human hepatoma cell lines, Hep3B, PLC/PRF/5 and HepG2, were characterised by a predominant transcription of only two (H2 and L) among the four genes involved in the synthesis of inter‐α‐trypsin inhibitor (ITI)‐related proteins. Pulse‐chase experiments followed by immunoprecipitation with specific anti‐L and anti‐H ITI antisera showed that the proteins synthesised displayed a restricted L and/or H2 antigenic reactivity. Furthermore, while Hep3B and PLC/PRF/5 lines only synthesised ITI precursors (mainly the L‐form), HepG2 cells were able to secrete an ITI‐like protein. Immunocytochemical analyses substantiated these results with uneven distribution of heavy and light‐chain polypeptide reactivity among the cells. The use of hepatoma cell models for the study of protein synthesis and assembly must therefore be considered cautiously.

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