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Gene expression and oligodendrocyte development in the myelin deficient rat

✍ Scribed by Dr. N. L. Nadon; I. D. Duncan

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 898 KB
Volume: 41
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490410111

No coin nor oath required. For personal study only.

✦ Synopsis

The proteolipid proteins play a major role in the structure of the CNS myelin sheath, but they have also been implicated in the oligodendrocyte development leading to myelination. Mutations in the PLP gene result in severe dysmyelination and a paucity of mature oligodendrocytes. The myelin deficient (md) rat, carrying a Thr75+Pro substitution present in both isoforms of proteolipid protein (PLP and DM20), is the most severely affected of the PLP mutants described to date. The expression of myelin associated genes was quantitated to determine the effect of the mutation on oligodendrocyte development in vivo. At 5 days postnatal, gene expression in the md rat approximated that in age-matched control rats, but as they matured, there was a progressive inhibition of gene expression in the md rats. The genes expressed late in the myelination program (PLP and MBP) were affected more dramatically than those expressed earlier in oligodendrocyte development (CNP and GPDH). The results indicate that the later stages of oligodendrocyte maturation and myelin elaboration are inhibited.

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