All five tryptophan biosynthetic genes of Saccharomyces cerevisiae were unified on plasmid pME554, which is based on 2 micrometer DNA and pBR322 sequences allowing for autonomous replication in yeast and E. coli. Homologous and heterologous expression of this artificial yeast TRP-gene cluster was st
Gene expression and engineering in yeast and other fungi
β Scribed by Patricia Tekamp-Olson; Pablo Valenzuela
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 894 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0958-1669
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A gene dosage effect was observed for esterase D in a patient with trisomy 13. Isoelectric focusing of hemoglobin from the same patient showed three unidentified bands. A gene dosage effect for several other enzymes was not observed in two other patients with trisomy 13.
We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula p o l y w p h a . Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 -lo4 pg DNA).
Eukaryotic translation initiation factors and their corresponding genes have been characterized using biochemical and genetic methods from a variety of different organisms. The designations of the factors relate to their apparent roles in the biochemical process. Many gene names indicate genetic int