Gene expression analysis of TFII-I modulated genes in mouse embryonic fibroblasts

✍ Scribed by Nyam-Osor Chimge; Ognoon Mungunsukh; Frank Ruddle; Dashzeveg Bayarsaihan

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 199 KB
• Volume: 308B
• Category: Article
• ISSN: 1552-5007
• DOI: 10.1002/jez.b.21134

✦ Synopsis

Abstract

TFII‐I is a founding member of a family of helix–loop–helix transcription factors involved in modulation of genes through interaction with various nuclear factors and chromatin remodeling complexes. Recent studies indicate that TFII‐I performs important function in cell physiology and mouse embryogenesis. In order to understand its molecular role, TFII‐I was overexpressed in primary mouse embryonic fibroblasts (MEFs) and alterations in gene expression were monitored with a mouse 16 K oligonucleotide microarray. These studies allowed us to identify genes that lie downstream of TFII‐I‐dependent pathways. Among the modulated candidates were genes involved in the immunity response, catalytic activity, signaling pathways and transcriptional regulation. Expression of several candidates including those for the interferon‐stimulated protein (G1p2), small inducible cytokine A7 (Ccl7), ubiquitin‐conjugating enzyme 8 (Ube2l6), cysteine‐rich protein (Csrp2) and Drosophila delta‐like 1 homolog (Dlk1) were confirmed by real‐time PCR. The obtained results suggest that TFII‐I participates in multiple signaling and regulatory pathways in MEFs. J. Exp. Zool. (Mol. Dev. Evol.) 308B:225–235, 2007. © 2006 Wiley‐Liss, Inc.