𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gene expression analysis in Interleukin-12-induced suppression of mouse mammary carcinoma

✍ Scribed by Xiaoyan Shi; Jianguo Liu; Zhaoying Xiang; Maki Mitsuhashi; Rita S. Wu; Xiaojing Ma

Publisher
John Wiley and Sons
Year
2004
Tongue
French
Weight
514 KB
Volume
110
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Interleukin‐12 (IL‐12) has potent antitumor activities via natural killer cells and cytotoxic T lymphocytes. However, the molecular mechanisms whereby IL‐12 induces tumoricidal activities are poorly understood. Here, we report the genome‐wide analysis of gene expression in a primary murine mammary carcinoma model that resembles human breast cancer, following the therapeutic application of recombinant IL‐12, which restricted tumor growth and metastasis. IL‐12 was able to curtail neovascularization in the tumor as well as enhance the number of tumor‐infiltrating lymphocytes. Comprehensive examination of global gene expression revealed IL‐12‐induced molecular changes associated with tumor regression and reduced lung metastasis, thus providing a high‐resolution snapshot of a host response against a developing malignancy and a rich source of potential targets for therapeutic intervention of breast cancer. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Retinoic acid induces expression of the
Retinoic acid induces expression of the interleukin-1β gene in cultured normal human mammary epithelial cells and in human breast carcinoma lines
✍ Limin Liu; Lorraine J. Gudas 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 286 KB 👁 1 views

## Abstract Retinoic acid (RA) and its derivatives inhibit the proliferation of normal human mammary epithelial cells (HMEC) and some breast carcinoma lines by mechanisms which are not fully understood. To identify genes that mediate RA‐induced cell growth arrest, an HMEC cDNA library was synthesiz

In vivo electrogene transfer of interleu
In vivo electrogene transfer of interleukin-12 inhibits tumor growth and lymph node and lung metastases in mouse mammary carcinomas
✍ Masa-Aki Shibata; Yuko Ito; Junji Morimoto; Ken Kusakabe; Ryoji Yoshinaka; Yoshi 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 911 KB

## Abstract ## Background Human breast cancer metastasizes mainly to lymph nodes, lungs, liver, and bone; in the majority of cases, it is the development of metastases which leads to death. In order to suppress mammary cancer metastasis, we applied __in vivo__ electrogene transfer (non‐viral metho

Analysis of interleukin receptor gene ex
Analysis of interleukin receptor gene expression in mouse fetal liver by quantitative polymerase chain reaction
✍ Gui-Quan Jia; Jose C. Gutierrez-Ramos 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 548 KB

We describe a simplified and sensitive polymerase chain reaction (PCR)-based method for the quantification of low-abundance RNA for mouse cytokine receptor genes. Accurate quantification is achieved in a two-step protocol which uses a synthetic RNA as an internal standard. The proper titration of th

Cell cycle analysis of foreign gene (β-g
Cell cycle analysis of foreign gene (β-galactosidase) expression in recombinant mouse cells under control of mouse mammary tumor virus promoter
✍ Man Bock Gu; Paul Todd; Dhinakar S. Kompala 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 879 KB

The cell cycle dependency of foreign gene expression in recombinant mouse L cells was investigated. Two different recombinant mouse L cell lines having the glucocorticoid receptor-encoding gene and the lacZ reporter gene were used in this study. The lacZ gene expression was controlled by the glucoco