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Gene dosage mutants at adenine phosphoribosyltransferase locus induced by colcemid in Chinese hamster V79-AP4 cells

✍ Scribed by Carlo M. Colella; Silvana Simi; Giuseppe Rainaldi; Theo van Boxel; Giuliano Della Valle; Antonio F. Peverali; Daniela Talarico; Tullio Mariani; Antonio Piras; Marcella Simili


Book ID
112557880
Publisher
Springer
Year
1988
Tongue
English
Weight
912 KB
Volume
14
Category
Article
ISSN
1572-9931

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To investigate the induction of gene amplification by a transient inhibition of DNA synthesis, V79-AP4 Chinese hamster cells were treated with 1-beta-D-arabinofuranosylcytosine (araC). At given intervals after the treatment, the frequency of N-(phosphonacetyl)-L-aspartate (PALA)-resistant colony-for