Gene dosage at theamylose-extenderlocus of maize: Effects on the levels of starch branching enzymes

✍ Scribed by Karen D. Hedman; Charles D. Boyer

• Book ID: 104784199
• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 468 KB
• Volume: 20
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00484699

✦ Synopsis

Soluble starch branching enzymes and starch synthases from maize kernels of differing dosage of the ae locus were purified by DEAE-cellulose chromatography. A near-linear relationship between increasing dosage of the dominate amylose-extender allele (Ae) and branching enzyme lib activity was found. In contrast, levels and properties of branching enzymes I and IIa, as well as the citrate-stimulated and primer-requiring starch synthases, remained unchanged. The near-linear increase in branching enzyme lib activity with increasing doses of the Ae allele is consistent with the hypothesis that ae is the structural gene coding for branching enzyme lib.