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Gene design of signal sequence for the effective secretion of recombinant protein using insect cell

โœ Scribed by Tsuchiya, Y.

Book ID
127030839
Publisher
Oxford University Press
Year
2005
Weight
173 KB
Volume
49
Category
Article
ISSN
0261-3166

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Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage sit