Gene cloning and heterologous synthesis of a haloalkaliphilic extracellular protease ofNatrialba magadii(Nep)

A multipurpose broad host range cloning

A multipurpose broad host range cloning vector and its use to characterise an extracellular protease gene of Xanthomonas campestris pathovar campestris

✍ Liu, Yu-Ning ;Tang, Ji-Liang ;Clarke, Belinda R. ;Dow, J. Maxwell ;Daniels, Mich 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 937 KB

A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 bp PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombi