Gene-chip analysis of the effect of tripeptide tyroservatide (YSV) on gene-expression in human hepatocarcinoma BEL-7402 tumors transplanted to nude mice

✍ Scribed by Jing Jia; Rong Lu; Chunlei Zhou; Lan Zhao; Zheng Fu; Zhitong Zhu; Li Wang; Minna Zheng; Jing Yang; Yanjun Zhang; Zhi Yao

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 131 KB
• Volume: 45
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.20172

✦ Synopsis

Abstract

Tyroservatide (YSV) is a bioactive tripeptide of tyrosyl‐seryl‐valine. In this study, we studied the effects of YSV on human hepatocarcinoma BEL‐7402 tumors transplanted in BALB/c (nu/nu) nude mice, and gene expression in the tumor cells with gene‐chip analysis. Results show that YSV significantly inhibits the growth of transplanted human hepatocarcinoma BEL‐7402 in nude mice (n = 12) compared with the control group (P < 0.05); with an inhibition rate of 55% at 320 µg/kg/d. Seven hundred eighty‐one genes were different between the YSV group and the control group. Fifty‐two genes changed in expression level by onefold or more including 37 downregulated genes and 15 upregulated genes. Probably, YSV exhibits a significant antitumor activity by inhibiting the expression of tumor cells histone genes, then damaging tumor cell chromosome and killing tumor cells. © 2005 Wiley‐Liss, Inc.