The ontogeny of lactate dehydrogenase (LDH) isozymes was examined in avian hybrids and compared with the isozyme patterns of the parental species. Hybrids were obtained by crossing female Japanese quail (Coturnix coturnix japonica) with male domestic chickens (Gallus gallus domesticus). By use of st
Gene activation of alcohol dehydrogenase in Japanese quail and chicken-quail hybrid embryos
โ Scribed by J. P. LeVine; L. E. Haley
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 715 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0006-2928
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โฆ Synopsis
No preferential activation of the maternally derived alcohol dehydrogenase (ADH) allele was found in any of the chicken male x Japanese quail female hybrids examined. ADH activity in the liver was, in fact, found to exist in two different cathodal zonal regions on starch gel electropherograms; the zone II bands appeared at day 5 of incubation in the quail embryo (day 6 in the hybrid embryo) and the zone I bands appeared in 9-day quail embryos (10-day hybrid embryos). By day 13 of incubation, only the faster-migrating zone I bands could be detected in both quail and hybrid embryos.
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The ontogeny of phosphoglueonate dehydrogenase in Japanese quails and chicken-quail hybrids was determined by vertical starch gel eleetrophoresis of pooled early embryos. The enzyme of maternal origin was found to be present at all stages of development. Paternal phosphogluconate dehydrogenase in pu
The initial appearance of a number of enzymes involved in gluconeogenesis was investigated in the early embryogenesis of the Japanese quail (Coturnix coturnix japonica), the domestic chicken (Gallus gallus domesticus), and chicken-quail hybrids. Starch gel electrophoresis and enzyme-specific stains