GENDER-RELATED DIFFERENCES IN ADHESION, GROWTH AND DIFFERENTIATION OF VASCULAR SMOOTH MUSCLE CELLS ARE ENHANCED IN SERUM-DEPRIVED CULTURES
✍ Scribed by L Bačáková; V Mareš; V Lisá
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 890 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
In 1-day cultures with 10% serum, the number of rat aortic smooth muscle cells (VSMC) adhering to the growth support was similar in cells from both sexes, whereas in 1% serum, the number of VSMC from male donors was lower. In 10% serum medium, the doubling time was significantly shorter and the number of [3H]thymidine-labelled nuclei was higher in cells of high passage from male rats. In serum-free medium, these differences increased and were also seen in cells of low passage number. Morphologically, the cells in male-derived cultures at higher passage number were mainly spindle-shaped, formed well-developed 'hills and valleys' and possessed longitudinally oriented bundles of alpha-actin-containing microfilaments. Most cells from female rats were flat, polygonal, the multilayered 'hills' were less prominent, with alpha-actin microfilaments forming a mesh-like network.