Gender differences in hepatic expression of flavin-containing monooxygenase isoforms (FMO1, FMO3, and FMO5) in Mice

✍ Scribed by Falls, J. Greg ;Blake, Bonnie L. ;Cao, Yan ;Levi, Patricia E. ;Hodgson, Ernest

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 692 KB
• Volume: 10
• Category: Article
• ISSN: 0887-2082
• DOI: 10.1002/jbt.2570100308

✦ Synopsis

Hepatic flavin-containing monooxygenase (FMO) activity of microsomes from adult CD-1, Swiss-Webster, C57BL/6, and DBA/2 mice was found to be significantly higher in females than in males. Based on protein and mRNA levels in CD-1 mice, FMO forms responsible for the gender difference in FMO activity were FMOl and FM03. FMOl expression was two to three times higher in female mice compared with males; FM03, however, which was expressed at levels equivalent to FMOl in female mice, was not detected in males. The expression of FM05 was approximately equal in both sexes. FMO2 and FM04 transcripts were not evident in hepatic mRNA from mice. Protein and mRNA levels appear to be coregulated with regard to gender-selective or gender-specific expression of FMOl or FM03, respectively. FM05, which demonstrates no gender-selective expression in mice, may be regulated by different mechanisms. Examination of protein levels among Swiss-Webster, C57BL/6, and DBA/2 strains revealed a gender-dependent expression of FMO isozymes identical to the CD-1 strain.