Gelatin Hydrogel Prepared by Photo-initiated Polymerization and Loaded with TGF-β1 for Cartilage Tissue Engineering
✍ Scribed by Xiaohong Hu; Lie Ma; Chengchao Wang; Changyou Gao
- Book ID
- 102474289
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 476 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Gelatin is a nature‐derived protein having good cytocompatibility, and widely used in tissue engineering particularly in a form of a hydrogel. To obtain the hydrogel with good enough mechanical properties, however, measures are still need to be taken. In this work, the gelatin molecule was modified with methacrylic acid (MA) to obtain crosslinkable gelatin (GM), which formed a chemically crosslinked hydrogel by photoinitiating polymerization. The gelation time could be easily tuned and showed an inverse relationship with the GM concentration. After photo‐irradiation for 20 min there was no detectable double carbon bond in the hydrogen spectrum of high resolution magic angle spinning nuclear magnetic resonance spectroscopy (^1^H HR‐MAS NMR). With the increase of the GM concentration, storage modulus and loss modulus of the hydrogels increased, but their swelling ratio and mesh size decreased. Weight loss of the hydrogels was also affected by the polymer concentration. Transform growth factor‐__β__1 (TGF‐__β__1) was incorporated into the GM hydrogel to improve its bioactivity. In vitro chondrocyte culture showed that the GM hydrogel had indeed good performance to support chondrocyte growth and maintain chondrocytic phenotype. Incorporation of TGF‐__β__1 could further improve the biological activity in terms of cell proliferation and extracellular matrix secretion.
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