Gel permeation chromatography using controlled-porosity glass. I. Polyacrylamide–formamide solution

✍ Scribed by Nobuhiko Onda; Kunio Furusawa; Noriko Yamaguchi; Shūgi Komuro

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 346 KB
• Volume: 23
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1979.070231218

✦ Synopsis

Abstract

The process of characterizing polyacrylamide and its partially hydrolyzed materials by gel permeation chromatography was examined. The use of controlled‐porosity glass and formamide as the stationary phase and the eluent, respectively, resulted in chromatographic behavior in accord with the hydrodynamic volume concept for polyacrylamide fractions. The addition of a salt (KCl) to the eluent was found to retard the elution of the hydrolyzed polyacrylamide.