Gel permeation chromatography on polyelectrolytes in aqueous solution

✍ Scribed by Jacques Desbrieres; Jean Mazet; Marguerite Rinaudo

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 278 KB
• Volume: 18
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(82)90119-7

✦ Synopsis

Gel permeation chromatography of water-soluble polymers, with special attention to polyelectrolytes, has been studied. A three-detector system (refractometry, conductimetry, viscometry) is used to characterize the polymers. A single curve is obtained for the calibration of the set of columns taking into account the hydrodynamic volume of the solutes and using a 0.1 N salt solution as the eluent to screen electrostatic forces. The role of the low adsorption of dextran on silica gel is pointed out. The dependence of elution volume on polymer concentration is demonstrated and interpreted in terms of the screening length of the molecules.