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Gel filtration of deoxyribonucleoprotein on agarose

✍ Scribed by J.E. Loeb


Book ID
115742977
Publisher
Elsevier Science
Year
1968
Weight
166 KB
Volume
157
Category
Article
ISSN
0005-2787

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A method is described which allows the obtention of total DNA from cells of Chlamydomonas reinhardi in 1 day. It is based on the gel filtration on agarose of crude lysates incubated for 1 hr with pronase and subsequently brought to 2 M NaCl in order to dissociate nucleoprotein complexes. The DNA ob