Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate

✍ Scribed by Katharine Gilbert; Kenneth Kustin; Guy C. McLeod

• Book ID: 102309302
• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 294 KB
• Volume: 93
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040930217

✦ Synopsis

Abstract

Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were unaffected by temperature from 4–21°C, and by ionic strength in the range 0.09‐1.0 M (NaCl). Appreciable loss of vanadium in the supernatant was experienced above pH 2.2. Experiments at pH 2.1 under anaerobic conditions show that the green chromogen and the vanadium‐containing bands elute separately. Under these experimental conditions, the vanadium‐containing species is of relatively low (⋍ 1,000) molecular weight.