Gel-entrapped penicillin G acylase optimized by an enzyme thermistor

✍ Scribed by A. Welwardová; P. Gemeiner; E. Michálková; L. Welward; A. Jakubová

• Book ID: 104645382
• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 363 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00153750

✦ Synopsis

Direct activity determination by a flow-through microcalorimetry in the enzyme thermistor system was employed for a fast comparison of (poly)acrylamide gel-entrapped penicillin G acylase preparations. Composition of the pre-polymerization cocktail and both the storage and operational stabilities of optimal gel-entrapped enzyme preparations isolated from the Escherichia col i industrial strain were optimized by this method. The validity of the results was corroborated by spectrophotometric measurements.