✦ LIBER ✦

Gel electrophoresis of fluorescent labeled cyanogen bromide cleavage products at the submicrogram level

✍ Scribed by Selina Chen-Kiang; Stanley Stein; Sidney Udenfriend

Book ID: 102984321
Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 750 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90194-5

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✦ Synopsis

A simple method is described for visualizing submicrogram amounts of the cyanogen bromide cleavage products of proteins on sodium dodecyl sulfate-polyacrylamide gels. The peptide fragment mixture is conjugated with the fluorogenic reagent 2-methoxy-2,4,diphenyl-3-(2H)furanone prior to electrophoresis. The fluorescent peptide bands are visible under ultraviolet light, thus avoiding the need for fixation and staining. The determination of the structural homology of two immunologically related proteins is presented to illustrate this methodology.

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