Gel electrophoresis : DNA Science Without the DNA!

✍ Scribed by Timothy Ter Ming Tan; Zong Ying Tan; Wei Liang Tan; Peng Foo Peter Lee

• Publisher: The American Society for Biochemistry and Molecular Biology
• Year: 2007
• Tongue: English
• Weight: 160 KB
• Volume: 35
• Category: Article
• ISSN: 1470-8175
• DOI: 10.1002/bmb.83

✦ Synopsis

Abstract

The aim of this project is to develop a simple system for the teaching and demonstration of DNA gel electrophoresis. DNA gel electrophoresis requires the use of specialized apparatus, toxic reagents, expensive agarose gel, and DNA samples, as well as a considerable amount of valuable classroom time to complete. A systematic evaluation of suitable alternative materials and components for the simulation of DNA gel electrophoresis was undertaken. A tried and tested set of combinations is presented here for educators to use in hands‐on classroom teaching, which does not require DNA, agarose, or Tris–borate–EDTA buffer. The use of common biological stains in place of DNA samples, agar–agar‐based gels, and weak electrolyte solutions provides a simple, inexpensive, and highly reproducible system that is adaptable to instructional needs. The migration of multicolored bands during electrophoresis provides an intuitive, compelling demonstration of the concept of electrophoresis.