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Geentic organization of the E. coli chromosome around the structural gene for initiation factor IF3 (infC)

โœ Scribed by Springer, M. ;Graffe, M. ;Grunberg-Manago, M.

Book ID
104747845
Publisher
Springer
Year
1979
Tongue
English
Weight
837 KB
Volume
169
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

A set of 2 transducing phages carrying varying lengths of the E. coli chromosome around the structural gene for initiation factor IF3 (infC) was derived from 2p2 which is known to carry, besides infC, the structural genes for the c~ subunit of phenylalanyl-tRNA synthetase (pheS), the fl subunit of phenylalanyl-tRNA synthetase (pheT) and the structural gene for threonyl-tRNA synthetase (thrS). The E. coli coding content of these derived phages was analysed by genetic complementation of a set of mutants and by SDS-polyacrylamide gel analysis of the proteins synthesized in UV irradiated cells infected with these phages. The segregation pattern of the different genes among these derived phages indicates that the order of the genes is pheT -pheS -"'P12" ' -(infC, thrS) where infC is probably between "P12" and thrS. "'P12" is the structural gene of a 12,000 molecular weight unidentified protein.

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โœ Brombach, Martin ;Pon, Cynthia L. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 794 KB

The expression of infC, the structural gene for translational initiation factor IF3, has been studied in different constructs under the control of the lambda PL and tac promoters. The amount of synthesized IF3 has been determined by a quantitative functional test and the levels of IF3-specific mRNA