GD3+ cells in the adult rat optic nerve are ramified microglia rather than 0–2Aadult progenitor cells
✍ Scribed by Dr. Guus Wolswijk
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 701 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0894-1491
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✦ Synopsis
The adult central nervous system (CNS) contains a population of adult oligodendrocyte-type-2 astrocyte (0-2A) progenitor cells (0-2Aud"" progenitor cells). These cells may provide a source of the new oligodendrocytes that are needed to repair demyelinated lesions. In order to examine the role of O-2AUd"'* progenitor cells in the regeneration of the oligodendrocyte population following demyelinating damage, it is essential to be able to identify such cells unambiguously in sections of adult CNS tissue. The present study examined whether antibodies to the ganglioside GD3 specifically latiel 0-2AUdu1' progenitor cells in cultures and sections of adult optic nerve, since previous studies on the developing CNS had suggested that O-2APerrnuta1 progenitor cells were GD3+ in vitro and in vivo. Evidence is presented indicating that, although O-2Aud"1t progenitor cells in vitro were labelled with the R24 mAb (an anti-GD, mAb), all cells in sections of adult optic nerve bound the OX-42 mAb and the B, isolectin derived from Griffonia Simplicifolia, and thus were not O-2Aud"'t progenitor cells, but ramified microglia. The data suggest that 0-2A"""" progenitor cells become GD3+ when placed in culture and that ramified microglia lose G,,-expression in vitro. o 1994 Wiley-Liss, Inc 0-2ApC?rC7lU t d