GC–SIM/MS Profiling of Urinary Steroids as their Per-trimethylsilyl Derivatives

✍ Scribed by M. N. Bakola-Christianopoulou; K. K. Apazidou; L. Psarros

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 132 KB
• Volume: 11
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199703)11:3<205::aid-aoc542>3.0.co;2-t

✦ Synopsis

Complete GC-SIM/MS separation and identification of 19 steroids (including several steroid isomers) in a male urine sample, as their per-trimethylsilyl ethers, is described. Trimethylsilylation of their functional groups (hydroxyl and carbonyl) was achieved by a rapid, simplified and one-step derivatization method, using N-methyl-N-trimethylsilyl-2,2,2,-trifluoroacetamide as the silylating agent and the solvent as well, along with catalytic amounts of trimethylsilyl iodide and dithioerythritol. Furthermore, a GC/MS method for the analysis of a synthetic mixture of underivatized urinary steroids is proposed.