Gas chromatographic determination of 6-deosvhesoses, pentoses alld hesoses o~cardiac glvcosides. I. Gas-liquid chromatography on packed columns
A new, relatively simple and accurate method allows the identification and quantification of the monosaccharide components of cardiac glycosides. After hydrolysis under standard conditions the trimethylsilyl ethers of digitoxose, cymarose, fucose, rhamnose, 6-deoxyallose, 6-deoxygulose, 6-deoxyglucose, 6deoxyidose, 6-deoxytalose, 3-0-methylglucose, allose, altrose, gulose, idose, glucose, mannose, galactose, talose, arabinose, lyxose, ribose and xylose can be identified by gas chromatography on OV-101 or OV-17 as stationary phases. The retention times of the main peaks of each of the analysed monosaccharides, and the ratios of the peak areas of the different anomers were found to be characteristic and of good reproducibility. With phenyl-B-D-glucopyranoside as internal standard the quantification of the absolute amount of sugar present in cardiac glycosides of known constitution is possible, the recovery after hydrolysis being 7 l-92 %. The method allows determination of the composition of the sugar chain in new cardiac glycosides and gives better information in a shorter time of analysis than other methods.