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Gas-liquid chromatography of the N(O)-heptafluorobutyrates of the isoamyl esters of amino acids : I. Separation and quantitative determination of the constituent amino acids of proteins

โœ Scribed by J.P. Zanetta; G. Vincendon

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
713 KB
Volume
76
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

A quantitative gas-liquid chromatographic method has been developed for the analysis of amino acids. The method involves the conversion of free amino acids into their isosmyl N(O)-heptafluorobutyryl esters and chromatograplly on 3 % SE-3o-Gas-Chrom Q. The method can be applied to acid-hydrolysed proteins or glycoproteins.

It is extremely sensitive (0.1 ng) and all common protein amino acids can be accurately determined in a single chromatographic run with pipecolic acid as internal standard,

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