Gas-liquid chromatography of local anesthetics and related compounds

✍ Scribed by Henry M. Koehler; John J. Hefferren

• Publisher: John Wiley and Sons
• Year: 1964
• Tongue: English
• Weight: 267 KB
• Volume: 53
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600530709

✦ Synopsis

sample patterns 8, 14, and 22, respectively, require about 1.5, 2.0, and 2.5 men per day. The assay takes a little longer than a mouse convulsion test, but is many times moreaccurate.

Since we were not assaying serum insulin we were able to increase volumes, standard concentrations, and I1al-insulii concentration to provide adequate counting quantities when low specific activity commercially available iodo-insulin was used. If high specific activity iodo-insulin is used, the sensitivity of the test could be increased by reverting to the original volumes used by Grodsky (1).

As suggested by Grodsky (l), we started using NazSa in the salt-out step but changed to Na2S04 for operation at 24'. Frictional heat arising during centrifugation led us to the use of a refrigerated centrifuge. However, since NafiO4 crystallizes at 5-15', we returned to Na2SOI and 1 5 ' as our operating temperature.

We have found this test to be accurate, fast, inexpensive, and specific for natural insulin.