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Gas-liquid chromatography of free amino acids in the hyaloplasm of rat liver and kidney cells

✍ Scribed by Janusz Gabrys; Janusz Konecki

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
494 KB
Volume
121
Category
Article
ISSN
0003-2697

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✦ Synopsis

Gas-liquid chromatography of free protein amino acids has a high and verified reproducibility of quantitative and qualitative estimation: Cytoplasmic matrix obtained from the 105,OOOg supernatant fraction of rat liver and kidney cells was investigated. The ultracentrifuged product was passed through ion-exchange resin, thus isolating free amino acids from the eluate. After lyophilization the product was derivatized to N-trifluoroacetyl n-butyl esters and separated on the OV 17 + 210 and ethylene glycol adipate phases. Flame ionization detection was used. Comparison of liver and kidney tissues showed significant differences in the concentrations of the amino acids studied.

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## Abstract A procedure is described for the quantitative determination of amino acids in hepatic and brain tissue samples from the rat. Because the presence of certain matrix components in the tissue material led to interference with chromatographic analysis they were removed by a prechromatograph