Gas-liquid chromatography of d-biotin

✍ Scribed by V. Viswanathan F. P. Mahn; V. S. Venturella; B. Z. Senkowski

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 330 KB
• Volume: 59
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600590329

✦ Synopsis

method. As shown in the table, there is no statistical difference between the methods with respect to the means and variances.

After the HCI-citric acid solution is added to the crushed tablet, a pH of 0.9-1 is observed. Slight effervescence occurs but subsides in less than 1-2 min. The extraction of the undissociated salicylic acid and aspirin by chloroform is then accomplished. The aspirin and salicylic acid are extracted from the chloroform solution by the buffer solution. The whole experiment takes about 10 min.

To ensure complete extraction of salicylic acid and aspirin into the chloroform solution, some of the aqueous phase was treated with 1 N NaOH and tested for the absence of salicylate fluorescence. Also, to be assured of complete extraction of salicylic acid from the chloroformic solution, some of the organic phase was run on the sprctrophotometer to observe the absence of salicylic acid.