Gas chromatography of fluorinated fatty acids : I. Separation and identification of lower 2-fluorofatty acids

✍ Scribed by Herman Gershon; J.A.A. Renwick

• Publisher: Elsevier Science
• Year: 1965
• Tongue: English
• Weight: 375 KB
• Volume: 20
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97373-x

✦ Synopsis

Using a Pye Argon Chromatograph, a 4 ft. I % SE30 column at ZZ~", flash heater 270°, pressure 15 psi. the reproducibility of the method was tested. Twelve samples of a pregnanediol diacetate solution, each 4 ,ug in 40 ,ul, were pipetted on to cylinders, evaporated, and the batch assayed using the automatic device described. Peak height and area by triangulation were determined and gave: height, mean 71 & s.d. 3.0; area, 1330 -& s.d. 46 mm2. The experiment was repeated the following day using the same column conditions but the evaporated samples in open glass cylinders were added and removed from the flash heater manually. This gave precision : height, mean 65 & s.d. 3.9; area, mean 1250 5. s.d. 70 'mm2.

The apparatus has been used chiefly.for overnight runs.' Samples"'and standards are loaded in the evening and a separate time switch is set to stop the chart drive at the end of the run. The peaks are quantitated the following morning.