Gas chromatography-mass spectrometric characteristics and assay of tele-methylhistamine

✍ Scribed by Lindsay B. Hough; Philip L. Stetson; Edward F. Domino

• Book ID: 102984358
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 665 KB
• Volume: 96
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90553-0

✦ Synopsis

To date, te/e-methylhistamine, the product of enzymatic histamine methylation, has not been measured by a suitable method. Tefe-andpros-methylhistamine both react quantitatively with trifluoroacetic and heptafluorobutyric anhydrides at room temperature under anhydrous conditions to yield either mono-or bis-derivatives in the absence or presence of pyridine, respectively. The extraction of tissue homogenates, de~vati~tion, and analysis by gas chromatography-mass fragmentography permits the detection of I rig/ml tele-methylhistamine, using pros-methylhistamine as the internal standard. Rat brain contains te/emethylhistamine in a concentration similar to that of histamine (42 rig/g). The content of this amine in tissue has been compared with that obtained by other methods.