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Gas chromatographic separation of DNP amino acids and its application to the analysis of serum amino acids

✍ Scribed by Nobuo Ikekawa; Osamu Hoshino; Reiko Watanuki; Hajime Orimo; Takuo Fujita; Masaki Yoshikawa

Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
407 KB
Volume
17
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

A modified two-dimensional paper chromat
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✍ Frederick W. Pairent; Martin B. Williamson πŸ“‚ Article πŸ“… 1960 πŸ› Elsevier Science 🌐 English βš– 268 KB

The 0.05 M phthslatc buffer was preparecl by mixing 50 ml of 0.1 M potassium acicl phthalato and 45.45 ml of 0.1 N NsOH and diluting to IOO ml with CO,-free waferG. l \* All the DNP-amino acicls used in this study were preparccl by the method of SANGEIZ~ or that of LEVY AND CHTJNG'.

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## Abstract A method is presented for analysis of protein amino acids in five minutes, including derivatization. The chemical treatment involves reaction with ethyl chloroformate: if the composition of the reaction medium is optimum this reagent esterifies carboxylic groups simultaneously with deri