Gas chromatographic determination of β-phenethylbiguanide and its metabolite p-hydroxy-β-phenethylbiguanide in serum and urine

✍ Scribed by Mois Mottale; Charles J. Stewart

• Book ID: 104144400
• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 564 KB
• Volume: 106
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93834-2

✦ Synopsis

A gas chromatographic method is presented for the detection of P-phenethylbiguanide (PEBG) and its metabolite, p-hydroxy-P-phenethylbiguanide (p-OH PEBG).

The procedure is applicable for the determination of the drug and its metabolite in the serum and urine of rats. The detection limit is 0.2,~g PEBG and 0.5 ,ug p-OH PEBG per ml of serum or urine.

A time course study of blood concentration and elimination rate following intraperitoneal injection of 100 mg/kg of PEBG to normal rats was performed. p-PEBG was found to be present in the blood and the urine, p-OH PEBG was only detected in the urine. Twenty-four hours following intraperitoneal injection, the urine contained 32% of the administered dose. 2O'x as unaltered PEBG and 12 "/, as p-OH PEBG.