Ganglioside patterns and cholera toxin-peroxidase labeling of aggregating cells from the chick optic tectum
✍ Scribed by Engel, Edgar L. ;Wood, John G. ;Byrd, Frances I.
- Book ID
- 102959549
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 912 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0022-3034
No coin nor oath required. For personal study only.
✦ Synopsis
The ganglioside compositions of the chick optic tectum and aggregating tectal cell cultures were examined. Both showed similar trends in changes in ganglioside patterns during development. G D ~ and GDlb were the predominant gangliosides early in development, while G D ~~ and several other multisialogangliosides increased in relative amounts with increasing age in vivo and in uitro.
Four gangliosides were present early in development which have not previously been reported. These gangliosides are not present at later developmental times suggesting a possible role for them during the critical early stages of nervous tissue differentiation. Some differences were noted when comparing in vivo versus in uitro ganglioside patterns; these differences may possibly be due t o the lack of normal retinotectal connections in the cultures. Cytochemical studies on the localization of the presumed cholera toxin-peroxidase binding site G M ~ showed conjugate binding correlates with increasing levels of G M ~ in the cultures. In older cultures, the conjugate was uniformly localized on all cells and processes in the aggregates. The conjugate also bound to synaptic membranes and intensely stained the synaptic cleft. This latter observation suggests an enrichment of G M ~ in the synaptic cleft region.