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Ganglioside loading of cultured fibroblasts: a provocative method for the diagnosis of the GM2 gangliosidoses

✍ Scribed by Joel Charrow; Helen J. Binns


Book ID
115825410
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
676 KB
Volume
156
Category
Article
ISSN
0009-8981

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A fluorometric method for monitoring the
✍ Kwok-Kam Yeung; Albert J. Owen; Joel A. Dain πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 505 KB

A fluorometric method for monitoring the enzymic hydrolysis of the terminal galactose from GM,-ganglioside has been developed. The released galactose is oxidized with galactose dehydrogenase and NAD and the fluorescence of the product NADH measured. This method can detect as little as 0.1 nmol of ga