Gamma-irradiation of cacao (Theobroma cacaoL.) pollen: Effect on pollen grain viability, germination and mitosis and on fruit set
✍ Scribed by M. Falque; A. A. Kodia; O. Sounigo; A. B. Eskes; A. Charrier
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 447 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0014-2336
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✦ Synopsis
Theobroma cacao pollen fertilization capability was studied after 0, 50, 100,200, 500 and 1000 Gy gammairradiation. For all irradiation doses, no alteration of pollen grain viability and in vitro germination was observed. In situ, for all doses, pollen tubes penetrated into the styles and reached the ovules 20 hours after pollination. In vitro observations of the pollen grain nuclei after 20 hours incubation showed that pollen irradiation causes inhibition of the division of the generative nucleus. Fruit survival rate 30 days after pollination decreased as irradiation doses increased from 0 to 100 Gy, and over 100 Gy no fruit set was obtained. The possibility of using irradiated pollen as a method for obtaining haploid cacao plantlets is discussed.