✦ LIBER ✦

Gallium nitrate: effects on cartilage during limb regeneration in the axolotl,Ambystoma mexicanum

✍ Scribed by Tassava, Roy A. ;Mendenhall, Luciara ;Apseloff, Glen ;Gerber, Nicholas

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Volume: 293
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.10116

No coin nor oath required. For personal study only.

✦ Synopsis

Gallium nitrate, a drug shown to have efficacy in Paget's disease of bone, hypercalcemia of malignancy, and a variety of experimental autoimmune diseases, also inhibits the growth of some types of cancer. We examined dose and timing of administration of gallium nitrate on limb regeneration in the Mexican axolotl, Ambystoma mexicanum. Administered by intraperitoneal injection, gallium nitrate inhibited limb regeneration in a dose-dependent manner. Gallium nitrate initially suppressed epithelial wound healing and subsequently distorted both anterior-posterior and proximo-distal chondrogenic patterns. Gallium nitrate given at three days after amputation severely inhibited regeneration at high doses (6.25 mg/axolotl) and altered the normal patterning of the regenerates at low doses (3.75 mg/axolotl). Administration of 6.25 mg of gallium nitrate at four or 14 days prior to amputation also inhibited regeneration. In amputated limbs of gallium-treated axolotls, the chondrocytes were lost from inside the radius/ulna. Limbs that regenerated after gallium treatment was terminated showed blastema formation preferentially over the ulna. New cartilage of the regenerate often attached to the sides of the existing radius/ulna proximally into the stump and less so to the distal cut ends. J. Exp. Zool. 293:384-394, 2002.

📜 SIMILAR VOLUMES

Retinoid antagonists inhibit normal patt

Retinoid antagonists inhibit normal patterning during limb regeneration in the axolotl,Ambystoma mexicanum

✍ Del Rincón, Sonia V. ;Scadding, Steven R. 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 919 KB

## Abstract Retinoic acid (RA) has been detected in the regenerating limb of the axolotl, and exogenous RA can proximalize, posteriorize, and ventralize blastemal cells. Thus, RA may be an endogenous regulatory factor during limb regeneration. We have investigated whether endogenous retinoids are e

Cell division and ribonucleic acid synth

Cell division and ribonucleic acid synthesis during the initiation of limb regeneration in larval axolotls (Ambystoma mexicanum)

✍ Kelly, Daryl J. ;Tassava, Roy A. 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 795 KB

## Abstract Mitotic activity and RNA synthesis were examined in normal regenerating limbs and denervated non‐regenerating limbs of larval axolotls during the first six days after amputation. H^3^‐uridine incorporation into RNA was measured by autoradiography of deoxyribonuclease treated sections. G

Pattern regulation during regeneration o

Pattern regulation during regeneration of embryonically produced symmetrical forelimbs in the axolotl,Ambystoma mexicanum

✍ Tank, Patrick W. 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 768 KB

## Abstract Embryonic axolotls (Stages 32–34) underwent surgery during which a graft of flank tissue was transferred from a donor animal to the anterior margin of the forelimb field in a host animal. Double‐posterior forelimbs containing from one to eight digits developed. These limbs were amputate

Effects of tunicamycin on retinoic acid

Effects of tunicamycin on retinoic acid induced respecification of positional values in regenerating limbs of the larval axolotl, Ambystoma mexicanum

✍ Keith J. Johnson; Dr. Steven R. Scadding 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 773 KB

Abnormal limb regeneration in the short-

Abnormal limb regeneration in the short-toes mutant of the axolotl,Ambystoma mexicanum: Studies of age, level of amputation, and extracellular matrix

✍ Gassner, Kathleen M.; Tassava, Roy A. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 402 KB 👁 2 views

Limb regeneration in the short-toes axolotl is impaired. Our goal was to characterize the regeneration process in this mutant by histological and immunocytochemical methods. Previous research indicates that age and a defective basement membrane may be instrumental factors in short-toes axolotl regen

The duration of the effectiveness of vit

The duration of the effectiveness of vitamin A at causing proximodistal duplication in regenerating limbs of the axolotl,Ambystoma mexicanum, in relation to whole body retinoid levels

✍ Johnson, Keith J. ;Scadding, Steven R. 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 711 KB

## Abstract Urodele amphibians regenerate their limbs after amputation. Normally, only those parts of the limb distal to the plane of amputation are regenerated. However, retinoids are capable of inducing proximodistal (PD) duplication such that structures already present proximal to the plane of a