Galectin-9 expands immunosuppressive macrophages to ameliorate T-cell-mediated lung inflammation
✍ Scribed by Tomohiro Arikawa; Naoki Saita; Souichi Oomizu; Masaki Ueno; Akihiro Matsukawa; Shigeki Katoh; Keisuke Kojima; Keiko Nagahara; Minoru Miyake; Akira Yamauchi; Hirotsugu Kohrogi; Mitsuomi Hirashima
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 576 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Abstract
Galectin‐9 (Gal‐9) plays pivotal roles in the modulation of innate and adaptive immunity to suppress T‐cell‐mediated autoimmune models. However, it remains unclear if Gal‐9 plays a suppressive role for T‐cell function in non‐autoimmune disease models. We assessed the effects of Gal‐9 on experimental hypersensitivity pneumonitis induced by Trichosporon asahii. When Gal‐9 was given subcutaneously to C57BL/6 mice at the time of challenge with T. asahii, it significantly suppressed T. asahii‐induced lung inflammation, as the levels of IL‐1, IL‐6, IFN‐γ, and IL‐17 were significantly reduced in the BALF of Gal‐9‐treated mice. Moreover, co‐culture of anti‐CD3‐stimulated CD4 T cells with BALF cells harvested from Gal‐9‐treated mice on day 1 resulted in diminished CD4 T‐cell proliferation and decreased levels of IFN‐γ and IL‐17. CD11b^+^Ly‐6C^high^F4/80^+^ BALF Mϕ expanded by Gal‐9 were responsible for the suppression. We further found in vitro that Gal‐9, only in the presence of T. asahii, expands CD11b^+^Ly‐6C^high^F4/80^+^ cells from BM cells, and the cells suppress T‐cell proliferation and IFN‐γ and IL‐17 production. The present results indicate that Gal‐9 expands immunosuppressive CD11b^+^Ly‐6C^high^ Mϕ to ameliorate Th1/Th17 cell‐mediated hypersensitivity pneumonitis.