Galactosylation by use of β-galactosidase: Chemo-enzymatic syntheses of di- and trisaccharides

✍ Scribed by Wolfgang H. Binder; Hanspeter Kählig; Walther Schmid

• Book ID: 104204850
• Publisher: Elsevier Science
• Year: 1994
• Tongue: French
• Weight: 867 KB
• Volume: 50
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(01)89581-8

✦ Synopsis

The synthesis of various galactose containing disaccharides has been achieved by utilizing the transgalactosylation potential of Rgalactosidase from Aspergilfus oryzue. Thus. using p nitrophenyl-BPgalactoside 1 as galactosyl donor, thio-glucosides 2a-c. thio-galactosides 5a. 5b and pent-4-enyl-glucoside 2d have proved to be useful acceptors for the enzyme catalyzed disaccharide formation. We further demonstrated the synthetic potential of the disaccharides obtained by enzymatic synthesis in preparing trisaccharide 10 starting with 4a and 4d and applying chemical glycosylation procedums developed for thio-and pent-4-enyl glycosides.