Galactose metabolism in gal mutants of Salmonella typhimurium and Escherichia coli
β Scribed by R.W.J. Hommes; W.A.M. Loenen; O.M. Neijssel; P.W. Postma
- Book ID
- 109313429
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 265 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0378-1097
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A genetic study of mutants deficient in hydrogenase activity was performed. In E. coli, the affected gene (hyd) is located at 51 min, between cys C and nal B; in S. typhimurium, it probably lies in the homologous region of the chromosome.
From libraries of EcoRI fragments of Salmonella typhimurium and Escherichia coli DNA in lambda gt7, phages could be isolated that carry mglB, the structural gene of the galactose-binding protein as well as other mgl genes. Lysogenization of an E. coli mutant carrying a defective galactose-binding pr