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G protein Gating of a Kir3.1-Prokaryotic Kir Channel Chimera Functionally Reconstituted in Planar Lipid Bilayers

✍ Scribed by Leal-Pinto, Edgar; Gómez-Llorente, Yacob; Sundaram, Shobana; Tang, Qiong-Yao; Ivanova-Nikolova, Tatyana; Mahajan, Rahul; Baki, Lia; Zhang, Zhe; Chavez, Jose; Ubarretxena-Belandia, Iban; Logothetis, Diomedes E.

Book ID: 123473095
Publisher: Biophysical Society
Year: 2011
Tongue: English
Weight: 57 KB
Volume: 100
Category: Article
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.2541

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## Abstract An affinity column prepared with noxiustoxin (NTx), a K^+^ channel blocker from the venom of the Mexican scorpion __Centruroides noxius__, was used to purify a functional channel from a detergent extract of Schwann cell membrane of the giant axon of the squid __Loligo vulgaris__. The pu