[G-3H]dansyl chloride in the analysis of γ-glutamyl-ε-lysine: A cautionary note
✍ Scribed by R.F. Venn; J.M. Basford; C.C. Curtis
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 283 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
G-3H]Dansyl Chloride in the Analysis of y-Glutamyk-lysine:
A Cautionary Note
In order to quantitatively analyze y-glutamyl-r-lysine, [G-3H]dansyl chloride was used to label [U-'*C]leucine and the isopeptide. After thin-layer chromatography of the products, the dansylated amino acid and isopeptide were acid hydrolyzed for up to 18 hr. The tritium was found to be labile under these conditions, with a half-life of exchange of about 5 hr, and the system behaved as if two labile tritium atoms were exchanging with pseudo-first-order rate constants of 0.5 and 0.05 hr-'. The errors introduced by acid hydrolysis render this reagent unsuitable for quantitative analysis.