Time-resolved fluorescence spectroscopy and global data analysis techniques have been used to study the flow of excitations in antennae of the green photosynthetic bacteria Chloroflexus aurantiacus and Chlorobium vibrioforme f. thiosulfatophilum. The transfer of energy from bacteriochlorophyll (BChl
Förster energy transfer in chlorosomes of green photosynthetic bacteria
✍ Scribed by Timothy P. Causgrove; Daniel C. Brune; Robert E. Blankenship
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 584 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1011-1344
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✦ Synopsis
Energy transfer properties of whole cells and chlorosome antenna complexes isolated from the green sulfur bacteria Chlorobium limicola (containing bacteriochlorophyll c), Chlorobium vibrioforme (containing bacteriochlorophyll d) and Pelodictyon phaeoclathratiforme (containing bacteriochlorophyll e) were measured. The spectral overlap of the major chlorosome pigment (bacteriochlorophyll c, d or, e) with the bacteriochlorophyll a B795 chlorosome baseplate pigment is greatest for bacteriochlorophyll c and smallest for bacteriochlorophyll e. The absorbance and fluorescence spectra of isolated chlorosomes were measured, fitted to gaussian curves and the overlap factors with B795 calculated. Energy transfer times from the bacteriochlorophyll c, d or e to B795 were measured in whole cells and the results interpreted in terms of the Förster theory of energy transfer.
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