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Fusion protein of adenovirus E4orf4 and human epidermal growth factor inhibits tumor cell growth

✍ Scribed by Yu Zhou; Hong Chen; Xiao-Li Ma; Hai-Jun Xie; Cun-Lin Wang; Sheng-Hua Zhang; Xin Wang; Bing-Ren Huang

Publisher
John Wiley and Sons
Year
2009
Tongue
French
Weight
285 KB
Volume
125
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Adenovirus early region 4 open reading frame 4 (E4orf4) protein is a novel cell death factor that selectively induces apoptosis in cancer cells. This study evaluated tumor inhibitory effects of a protein made by fusion E4orf4 and human epidermal growth factor (EGF). EGF was used to ensure the selective targeting of EGF receptor (EGFR)‐overexpressing tumor cells. Results showed that EGF‐E4orf4 stimulated EGFR phosphorylation in a time‐ and dose‐dependent manner. Confocal microscopy analysis showed both EGF‐E4orf4 and EGF could be internalized via EGFR but they had different intercellular trafficking pathways. In vitro study showed that EGF‐E4orf4 significantly inhibited the proliferation of BGC823 and i__n vivo__ study showed EGF‐E4orf4 suppressed tumor growth in a dose‐dependent fashion with an inhibition rate of 79% for MDA‐MB‐231 and 49% for BGC 823 (p < 0.05). No toxic effects were observed in the nude mice with a dose as high as 10 mg/kg of EGF‐E4orf4. These results indicated that EGF‐E4orf4 could be a potential drug for cancer therapy. © 2009 UICC

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