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Fusion expression of mutated cecropin CMIV inE. coli

✍ Scribed by Wei Xie; Qifeng Qiu; Xueyin Dong; Zichun Hua; Xianxiu Xu


Book ID
105639828
Publisher
Science in China Press (SCP)
Year
1997
Tongue
English
Weight
482 KB
Volume
40
Category
Article
ISSN
1674-7305

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Luciferase from Vibrio harveyi is encoded by t w o adjacent genes, luxA and IuxB. The t w o genes were fused by replacing a segment extending from near the end o f luxA into the N-terminal end of lux6 by a synthetic oligonucleotide. The construction removed the TAA stop codon at the end o f IuxA, th