Fused-silica microparticulate packed column HPLC-split flow gradient elution
✍ Scribed by Yang, F. J.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 425 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Fused-silica microparticulate packed columns are demonstrated to have equal or better separation efficiency and resolving power than conventional 4.6 mm i.d. packed columns. While neither separation efficiency nor resolving power is an inherent consequence of capillary column use, there are potential advantages of using fused-silica capillary columns, particularly when microparticulate particles of 1 to 5 pm are used in the gradient elution mode. Gradient elution techniques compatible with flow rates below 30 pL/min are discussed. Split-flow gradient elution of twenty PTH aminoacids using 10 and 40 cm x 0.54 mm i.d. columns packed with 4 pm Micro-Pak PTH-AA particles is demonstrated. Detection limits of 0.1 -0.3 picomoles are measured for PTH amino acids and 0.01-0.02 picomoles for tricyclic antidepressant drugs.