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Fusarin C Biosynthesis in Fusarium moniliforme and Fusarium venenatum

✍ Scribed by Zhongshu Song; Russell J. Cox; Colin M. Lazarus; Thomas J. Simpson FRS

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 340 KB
Volume: 5
Category: Article
ISSN: 1439-4227
DOI: 10.1002/cbic.200400138

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✦ Synopsis

Abstract

Fragments of polyketide synthase (PKS) genes were amplified from complementary DNA (cDNA) of the fusarin C producing filamentous fungi Fusarium moniliforme and Fusarium venenatum by using degenerate oligonucleotides designed to select for fungal PKS C__‐methyltransferase (C__MeT) domains. The PCR products, which were highly homologous to fragments of known fungal PKS C__MeT domains, were used to probe cDNA and genomic DNA (gDNA) libraries of__ F. moniliforme and F. venenatum. A 4.0 kb cDNA clone from F. venenatum was isolated and used to prepare a hygromycin‐resistance knockout cassette, which was used to produce a fusarin‐deficient strain of F. venenatum (kb=1000 bp). Similarly, a 26 kb genomic fragment, isolated on two overlapping clones from F. moniliforme__, encoded a complete iterative Type I PKS fused to an unusual nonribosomal peptide synthase module. Once again, targeted gene disruption produced a fusarin‐deficient strain, thereby proving that this synthase is responsible for the first steps of fusarin biosynthesis.__

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