Further studies on the separation of cyclic (1→2)-β-d-glucans (cyclosophoraoses) produced by rhizobium meliloti ifo 13336, and determination of their degrees of polymerization by high-performance liquid chromatography
✍ Scribed by Kyoko Koizumi; Yasuyo Okada; Toshiko Utamura; Makoto Hisamatsu; Akinori Amenura
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 562 KB
- Volume
- 299
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Eight pure cyclic (l-2)$-D-glucans (cyclosophoraoses) varying in size from 17 to 24 residues were previously isolated from culture filtrates of Agrobacterium and Rhizobium. Thereafter further studies on the separation of cyclosophoraoses by highperformance liquid chromatography (HPLC) with small-particle columns showed an occurrence of many cyclosophoraoses having degrees of polymerization (DPs) of more than 24. One sample prepared f om Rhizobium meliloti IF0 13336 contained 5?_ large amounts of higher cyclosophorao s of up to at least DP 40, which were separated clearly on a 3-pm chemically modified amine column (ERC-NH-1171). Some pure cyclosophoraoses with higher DPs were isolated by liquid chromatography using reversed-phase columns, and their DPs were determined by HPLC of their partial hydrolysates.